Comparing the Effects of Alpha-Tocopherol and Tocotrienol Isomers on Osteoblasts hFOB 1.19 Cultured on Bovine Bone Scaffold

Tocotrienol mixtures have been shown to exert anabolic actions on the skeletal system in animal studies, but it is unclear which tocotrienol isomer shows most prominent effects. This study aims investigate active isomers using hFOB 1.19 human osteoblasts cultured a bovine bone scaffold. The trabecular was sectioned, demineralised and freeze-dried form were scaffolds humidified condition at 37 °C 5% carbon dioxide with vitamin E (alpha-, beta-, gamma-, delta-tocotrienol alpha-tocopherol). cell differentiation capacity of investigated through morphological observation, alkaline phosphatase (ALP) activity osteocalcin expression. Changes determined histomorphometry methods. Osteoblast culture treated gamma- delta-tocotrienols showed significant increase ALP Bone structural analysis that increases volume thickness. In conclusion, show effects by increasing osteoblast enhancing microstructure.